Methods and means for efficient skipping of at least one of the following exons of the human duchenne muscular dystrophy gene: 43, 46, 50-53

An isolated antisense oligonucleotide which is fully complementary to 8-22 consecutive nucleotides of a sequence of an exon of human dystrophin pre-mRNA, said oligonucleotide comprising a locked nucleic acid, wherein said sequence of said exon is selected from the group consisting of:

• 5′-AGAUAGUCUACAACAAAGCUCAGGUCGGAUUGACAUUAUUCAUAG CAAGAAGACAGCAGCAUUGCAAAGUGCAACGCCUGUGG-3′ (SEQ ID NO:
• 2), and wherein said oligonucleotide is capable of skipping exon 43;
• 5′-UUAUGGUUGGAGGAAGCAGAUAACAUUGCUAGUAUCCCACUUGAA CCUGGAAAAGAGCAGCAACUAAAAGAAAAGC-3′ (SEQ ID NO:
• 3), and wherein said oligonucleotide is capable of skipping exon 46;
• 5′-GCGGTAAACCGUUUACUUCAAGAGCUGAGGGCAAAGCAGCCUGAC CUAGCUCCUGGACUGACCACUAUUGG-3′ (SEQ ID NO:
• 4), and wherein said oligonucleotide is capable of skipping exon 50;
• 5′-CUCCUACUCAGACUGUUACUCUGGUGACACAACCUGUGGUUACUA AGGAAACUGCCAUCUCCAAACUAGAAAUGCCAUCUUCCUUGAUG UUGGAGGUAC-3′ (SEQ ID NO:
• 5), and wherein said oligonucleotide is capable of skipping exon 51;
• 5′-AUGCAGGAUUUGGAACAGAGGCGUCCCCAGUUGGAAGAACUCAUU ACCGCUGCCCAAAAUUUGAAAAACAAGACCAGCAAUCAAGAGGCU-3′ (SEQ ID NO:6), and wherein said oligonucleotide is capable of skipping exon 52, and
• 5′-AAUGUUAAAGGAUUCAACACAAUGGCUGGAAGCUAAGGAAAAGCU GAGCAGGUCUUAGGACAGGCCAGAG-3′ (SEQ ID NO:7), and wherein
• said oligonucleotide is capable of skipping exon 53.