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US 2019/0100751 A1Published application
Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
AssigneeGen9, Inc.
InventorsJoseph Jacobson, Martin J. Goldberg, Li-Yun A. Kung, Daniel Schindler, Michael E. Hudson
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Abstract
Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.
Claims20
-30. (canceled)
A method for isolating a nucleic acid having a predefined sequence, the method comprising:
- (a) providing a pool of nucleic acid molecules comprising error-free and error-containing nucleic acid molecules;
- (b) tagging the nucleic acid molecules;
- (c) optionally fragmenting the nucleic acid molecules;
- (d) determining the sequence of the nucleic acid molecules;
- (e) localizing the error-free and error-containing nucleic acid molecules;
- and
- (f) isolating the error-free nucleic acid molecules, wherein the error-free nucleic acid molecules have a predefined sequence.
4 more elements
-39. (canceled)
Dependencies are inferred from the claim text.