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US 2019/0100747 A1Published application
Purification and detection of analytes
AssigneeCalifornia Institute of Technology
InventorsRustem ISMAGILOV, Erik Jue, Daan Witters
Life of this patent
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Abstract
A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.
Claims23
A device comprising:
- a plurality of chambers, each chamber of the plurality of chambers comprising a top opening, a bottom opening, and a seal;
- a lid configured to:
- seal the plurality of chambers by contacting the seal of each chamber of the plurality of chambers, and
- upon pushing of the lid towards the plurality of chambers, simultaneously pressurize each chamber of the plurality of chambers;
- a storage section comprising a plurality of receptacles;
- and
- a rotating section between the plurality of chambers and the storage section, the rotating section comprising a solid phase column, wherein the rotating section is configured to rotate to form, in sequence, a fluidic pathway between the solid phase column, the bottom opening of each chamber of the plurality of chambers, and a receptacle of the plurality of receptacles, thereby allowing flow of a pressurized fluid from a chamber of the plurality of chambers through the solid phase column into a receptacle of the plurality of receptacles.
4 more elements
A device comprising:
- a first plate comprising:
- a first fluidic channel having a first inlet and a first plurality of outlets;
- and
- a second fluidic channel having a second inlet and a second plurality of outlets;
- and
- a second plate comprising a first plurality of reaction wells and a second plurality of reaction wells,
- wherein:
- the second plate is configured to rotate, relative to the first plate, between a first position and a second position,
- the first position forms a fluidic pathway between each reaction well of the first plurality of reaction wells and an outlet of the first plurality of outlets, and a fluidic pathway between each reaction well of the second plurality of reaction wells and an outlet of the second plurality of outlets, and
- the second position fluidically isolates the first plurality of reaction wells from the first plurality of outlets, and the second plurality of reaction wells from the second plurality of outlets.
7 more elements
Dependencies are inferred from the claim text.