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US 2019/0100746 A1Published application
Use of an aqueous composition for dissolving biomolecules from a tissue sample
AssigneeAllflex Europe
InventorsJOHAN DE MEULEMEESTER, EMMANUEL LEMAIRE
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Abstract
The present invention relates to the use of an aqueous composition for dissolving biomolecules from a tissue sample and to a method of using an aqueous composition for dissolving biomolecules from a tissue sample. Furthermore, the present invention relates to a system for preparing a tissue sample of an animal.
Claims17
A method for dissolving biomolecules selected from nucleic acids and proteins, from a tissue sample of an animal and for subsequent
- a) preserving said biomolecules or
- b) further processing said biomolecules,
- wherein said method comprises the steps:
- obtaining a tissue sample of said animal and
- immediately after obtaining the sample, exposing said tissue sample to an aqueous composition by contacting said sample with said composition, said composition comprising:
- a buffer capable of buffering at a pH range from 7 to 9 at 25° C.,
- a detergent,
- a salt at a concentration of 5-10 wt. %,
- a chelating agent, and water,
- wherein said tissue of an animal is obtained using a tissue sampling tag or a tissue sampling biopsy needle, and wherein said sample is contacted with said composition by introducing said sample into said composition, and wherein by exposing said sample to said composition, biomolecules selected from nucleic acids and proteins, from said tissue sample become dissolved in said aqueous composition to produce a biomolecule solution,
- using said biomolecule solution for preserving said biomolecules or for further processing said biomolecules, and
- optionally using said aqueous composition to store said tissue sample for later use.
9 more elements
A system for preparing a tissue sample of an animal for subsequent
- a) genotyping said tissue,
- b) detection of a pathogen in said tissue, or
- c) storing and preserving said tissue sample for later use,
- said system comprising a tissue sampling tag or a tissue sampling biopsy needle, and an aqueous composition for dissolving biomolecules from a tissue sample of an animal, said composition being contained in a container, said composition comprising
- a buffer capable of buffering at a pH range from 7 to 9 at 25° C.,
- a detergent,
- a salt at a concentration of 5-10 wt. %,
- a chelating agent, and water.
5 more elements
Dependencies are inferred from the claim text.