Modified Strains for the Production of Recombinant Silk
AssigneeBolt Threads, Inc.
InventorsMatthew Scott Gamboa, Joshua Tyler Kittleson
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Abstract
Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains.
Claims50
A Pichia pastoris microorganism, in which the activity of a YPS1-1 protease and a YPS1-2 protease has been attenuated or eliminated, wherein said microorganism expresses a recombinant polypeptide.
A Pichia pastoris engineered microorganism comprising YPS1-1 and YPS1-2 activity reduced by a mutation or deletion of the YPS1-1 gene comprising SEQ ID NO:
- 1 and the YPS1-2 gene comprising SEQ ID NO:
- 2, wherein said microorganism further comprises a recombinantly expressed protein comprising a polypeptide sequence encoded by SEQ ID NO:
- 462.
A method of modifying Pichia pastoris to reduce the degradation of a recombinantly expressed protein, comprising knocking out or mutating a gene encoding a YPS1-1 protein and a YPS1-2 protein.
Dependencies are inferred from the claim text.