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US 2019/0100739 A1Published application
Methods for the manufacture of proteolytically processed polypeptides
AssigneeIpsen Bioinnovation Limited
InventorAndreas Rummel
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Abstract
The present invention relates to a novel protcolytically active polypeptide and various uses of the polypeptide (and others) in screening and manufacturing methods.
Claims16
1-8. (canceled)
A method for manufacturing a di-chain botulinum neurotoxin serotype F (BoNT/F) or derivative thereof, the method comprising contacting a Lys-C polypeptide with a single-chain BoNT/F or derivative thereof;
- wherein the single-chain BoNT/F or derivative thereof is proteolytically processed by the Lys-C polypeptide to produce a di-chain BoNT/F or derivative thereof.
11-13. (canceled)
15-19. (canceled)
A method of screening for an inhibitor of a Lys-C polypeptide, the method comprising:
- (a) contacting the Lys-C polypeptide with a substrate and a putative inhibitor;
- and
- (b) determining the effect of the putative inhibitor on the conversion of the substrate into cleavage product;
- wherein the substrate is a single-chain BoNT/F or derivative thereof and the cleavage product is a di-chain BoNT/F or derivative thereof;
- and
- wherein a reduction in the amount of the cleavage product relative to the amount produced when the Lys-C polypeptide is contacted with the substrate but not with the putative inhibitor is indicative of the inhibitory effect of the putative inhibitor.
3 more elements
21-22. (canceled)
Dependencies are inferred from the claim text.