A Liquid Formulation of Alpha-Amylase
AssigneeBASF Enzymes LLC
InventorYun HAN
Life of this patent
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Abstract
The present disclosure relates to liquid enzyme formulations containing one or more alpha-amylases for use in starch processing, wherein the pH of the enzyme formulation is about pH 6.0-8.0, and methods of use thereof. The present disclosure further relates to methods of making a liquid enzyme formulation containing one or more alpha-amylase having improved stability, comprising titrating the pH of the liquid enzyme formulation to a range of pH 6.0-8.0.
Claims24
A liquid enzyme formulation comprising:
- (a) an alpha-amylase;
- (b) a buffering agent;
- (c) a stabilizer;
- and
- (d) a preservative,
- wherein the pH of the enzyme formulation is about pH 6.0-8.0.
A method of making a liquid enzyme formulation comprising:
- (a) providing a composition comprising an alpha-amylase, wherein the composition has a pH value of about 8.0 to about 10.5;
- (b) adding a stabilizer to the composition comprising the alpha-amylase to obtain a liquid enzyme formulation;
- and
- (c) titrating the pH of the liquid enzyme formulation to a range of pH 6.0-8.0.
Use of a liquid enzyme formulation, comprising:
- (a) providing a liquid enzyme formulation comprising an alpha-amylase, a buffering agent, a stabilizer, and a preservative, wherein the pH value of the liquid enzyme formulation is about pH 6.0-8.0;
- (b) providing a starch;
- and
- (c) contacting the liquid enzymatic formulation with the starch, thereby hydrolyzing the starch.
A liquid enzyme formulation comprising:
- (a) an alpha-amylase;
- (b) a buffering agent;
- (c) a stabilizer;
- and
- wherein the pH of the enzyme formulation is about pH 6.0-8.0.
A liquid enzyme formulation comprising:
- (a) an alpha-amylase;
- wherein the alpha-amylase comprises:
- an amino acid sequence having at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to one of the amino acid sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:14;
- (b) a buffering agent;
- wherein the buffering agent comprises:
- sodium citrate, potassium citrate, citric acid, sodium acetate, acetic acid, sodium phosphate, potassium phosphate, or any combination thereof.
- (c) a stabilizer, wherein the stabilizer comprises:
- sucrose, sorbitol, mannitol, glycerol, trehalose, sodium chloride, sodium sulfate, or any combination thereof;
- and
- wherein the pH of the enzyme formulation is about pH 6.0-8.0.
Dependencies are inferred from the claim text.