Assay for the removal of methyl-cytosine residues from dna
AssigneeYissum Research Development Company of the Hebrew University of Jerusalem
InventorsAsaf HELLMAN, Nurit MERON
Life of this patent
Estimating the term…
Abstract
An isolated polynucleotide encoding a fusion protein which comprises a catalytically inactive CRISPR associated 9 (dCas9) protein linked to a TET protein is disclosed. Use thereof and of the fusion protein itself is also disclosed.
Claims22
1-21. (canceled)
A kit comprising:
- a polynucleotide encoding a fusion protein which comprises a catalytically inactive CRISPR associated 9 (dCas9) protein linked to a TET protein;
- and
- at least one guide RNA which is directed to an enhancer of a predetermined target gene.
(canceled)
A method of modifying DNA methylation of a target gene in a cell, the method comprising expressing a polynucleotide encoding a fusion protein which comprises a catalytically inactive CRISPR associated 9 (dCas9) protein linked to a TET protein in the cell, and one or more guide RNA directed to an enhancer of the target gene.
Dependencies are inferred from the claim text.