Ex-vivo intestinal culture model, methods of producing same and uses thereof
AssigneesThe State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organizat, Mor Research Applications Ltd.
InventorsHinanit KOLTAI, Yoram KAPULNIK, Marcelo FRIDLENDER, Timna NAFTALI
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Abstract
A method of preparing an ex-vivo intestinal culture model is provided. The method comprising: (a) providing an intestinal tissue sample; (b) treating the intestinal tissue sample with a protease cleaving an extracellular matrix (ECM) component so as to break cell-cell contacts and obtain an intestinal preparation which maintains the overall intestinal tissue structure including villi and/or intestinal tissue layer orientation; (c) washing the preparation; and (d) culturing the preparation.
Claims23
A method of preparing an ex-vivo intestinal culture model, the method comprising:
- (a) providing an intestinal tissue sample;
- (b) treating said intestinal tissue sample with a protease cleaving an extracellular matrix (ECM) component so as to break cell-cell contacts and obtain an intestinal preparation which maintains the overall intestinal tissue structure including villi and/or intestinal tissue layer orientation;
- (c) washing said preparation;
- and
- (d) culturing said preparation.
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Dependencies are inferred from the claim text.