Cells for Therapy of the Heart
AssigneeCharite-Universitatsmedizin Berlin
InventorsMarion Haag, Jochen Ringe, Michael Sittinger, Carsten Tschope
Life of this patent
Estimating the term…
Abstract
According to the invention fibroblast-like cells obtained from heart muscle biopsies, which are CD90 negative, CD105 positive, CD117 negative and/or CD166 positive as well as cell preparations of such cells for therapy of heart diseases as well as a method for providing the latter are disclosed. The cells according to the invention are characterized by a good cultivability in cell culture. Furthermore a method for obtaining the cells and cell preparations according to the invention are disclosed.
Claims21
A method for obtaining a cell preparation comprising isolated fibroblast-like cells, the method comprising:
- a) in a first cell culture step, culturing a tissue sample obtained from mammalian heart muscle tissue under conditions suitable for culture of mammalian cells in cell culture medium, in a cell culture container having a solid surface;
- b) detaching cells that have grown out of the tissue sample and that adhere to the solid surface in a passage step by means of limited proteolysis, isolating the detached cells;
- c) culturing the sorted cells in a second cell culture step;
- thereby obtaining the cell preparation that is CD90 negative;
- CD105 positive;
- CD106 negative and CD117 negative.
A method for treatment of cardiac disease, comprising
- administering to a subject in need thereof a therapeutically effective amount of a cell preparation comprising isolated fibroblast-like cells, obtained from mammalian heart muscle tissue, wherein the isolated fibroblast-like cells are CD90 negative;
- CD105 positive;
- CD106 negative and CD117 negative.
Dependencies are inferred from the claim text.