Cell culture medium and culture medium supplement
AssigneeInstitute of Biophysics, Chinese Academy of Sciences
InventorsYue Ma, Junjie Jiang, Fei Pei
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Abstract
In one aspect, the present disclosure relates to a substantially albumin-free, chemically defined medium for efficiently supporting stem cell differentiation with significantly improved reproducibility and long-term culture of the differentiated cells. In particular embodiments, provided herein are compositions and methods for promoting atrial and ventricular cardiomyocytes formation from stem cells. The present disclosure further relates to the atrial and ventricular cardiomyocytes formed from the stem cells, and the uses of the cardiomyocytes, e.g., for cardiac injury repairmen, cardiac safety evaluation during drug discovery, and screening for new therapeutics for treating cardiac injuries.
Claims36
A cell culture medium supplement comprising at least one antioxidant or at least two different antioxidants that substitute(s) the function of albumin in a cell culture medium, wherein said cell culture medium supplement is configured to be combined with a basal culture medium to form a substantially albumin-free cell culture medium.
A cell culture medium supplement comprising at least one antioxidant or at least two different antioxidants selected from the group consisting of:
- a) ascorbic acid, ascorbate, or a salt or an ester thereof,
- b) a water-soluble analog of vitamin E,
- c) N-acetyl-cysteine or glutathione, or a salt or an ester thereof,
- d) pyruvic acid, pyruvate, or a salt or an ester thereof,
- e) a catalase,
- f) a superoxide dismutase,
- g) a thiol, such as 2-mercaptoethanol or 1-thioglycerol,
- h) a metallothione,
- i) a thioredoxin,
- j) lipoic acid or a salt or an ester thereof,
- k) uric acid or a salt or an ester thereof,
- l) a carotene,
- m) melatonin,
- n) probucol,
- o) dimethylthiourea, and
- p) resveratrol,
- wherein said cell culture medium supplement is configured to be combined with a basal culture medium to form a substantially albumin-free cell culture medium.
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Dependencies are inferred from the claim text.