Cell culture medium, culture method, and organoid
AssigneesKeio University, Osaka University
InventorsToshiro SATO, Mami MATANO, Shinya SUGIMOTO, Junichi TAKAGI
Life of this patent
Estimating the term…
Abstract
The present invention provides a cell culture medium with which serum-free long-term culture of an epithelial stem cell, an epithelial cancer cell, or a tissue containing at least one thereof can be achieved. The cell culture medium of the present invention includes: a Wnt agonist composed of a complex of Wnt protein and afamin, which is a substance capable of stabilizing the Wnt protein, and R-spondin; and at least one selected from the group consisting of a mitogenic growth factor, a bone morphogenetic protein (BMP) inhibitor, a transforming growth factor-β (TGF-β) inhibitor, and a p38 inhibitor.
Claims16
A cell culture medium comprising:
- a Wnt agonist including a complex of a Wnt protein and an afamin, which is a substance capable of stabilizing the Wnt protein;
- and
- at least one selected from the group consisting of an R-spondin, a mitogenic growth factor, a bone morphogenetic protein (BMP) inhibitor, a transforming growth factor-β (TGF-β) inhibitor, and a p38 inhibitor.
Dependencies are inferred from the claim text.