Metastable state mixing

A method, comprising:

• drawing, via a reciprocating pump operated by a controller, a volume of a solution of spores from a spore container, a volume of a solution of nutrients from a nutrient container, and a volume of water through an arrangement of valves and tubes;
• expelling, via the reciprocating pump, the volume of the solution of spores, the volume of the solution of nutrients, and the volume of water from the reciprocating pump to a mixing device;
• mixing, via the mixing device, the volume of the solution of spores, the volume of the solution of nutrients, and the volume of water together into a mixture of the spores, the solution of nutrients, and the water;
• providing the mixture of the spores, the nutrients, and the water to a holding tank from the mixing device;
• and
• heating, via a heater operated by the controller, the mixture of the spores, the nutrients, and the water in the holding tank, wherein a thermocouple is to used measure a temperature of the mixture in the holding tank.