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US 2019/0100720 A1Published application
Flow through electroporation instrumentation
AssigneeInscripta, Inc.
InventorsJorge Bernate, Don Masquelier
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Abstract
The present disclosure provides a flow-through electroporation device configured for use as a stand-alone module or as one module in an automated multi-module processing system.
Claims20
A flow-through electroporation (FTEP) device for introducing an exogenous material into cells in a fluid, the FTEP device comprising:
- a. at least a first inlet and at least a first inlet channel for receiving a fluid comprising cells and/or exogenous material into the FTEP device;
- b. an outlet and an outlet channel for removing a fluid comprising transformed cells and exogenous material from the FTEP device;
- c. a flow channel intersecting and positioned between the at least first inlet channel and the outlet channel, wherein the flow channel decreases in width to a dimension no smaller than at least 2× diameter of the cells being electroporated between the first inlet channel and a center of the flow channel and the outlet channel and the center of the flow channel;
- and
- d. two electrodes positioned in electrode channels between the intersection of the flow channel with the first inlet channel and the intersection of the flow channel with the outlet channel and on either end of where the flow channel decreases in width;
- wherein the electrodes are in fluid and electrical communication with fluid in the flow channel but not directly in the path of the cells traveling through the flow channel;
- and wherein the electrodes apply one or more electric pulses to the cells in the fluid as they pass through the flow channel, thereby introducing exogenous material into the cells in the fluid.
4 more elements
A flow-through electroporation (FTEP) device for introducing an exogenous material into cells in a fluid, the device comprising:
- at least one inlet and at least one inlet channel for introducing a fluid comprising cells and exogenous material to the FTEP device;
- an outlet and an outlet channel for removing transformed cells and exogenous material from the FTEP device;
- a flow channel positioned between a first inlet channel and the outlet channel, wherein the flow channel intersects with the first inlet channel and the outlet channel and wherein a portion of the flow channel narrows between the inlet channel intersection and the outlet channel intersection to a dimension no smaller than at least 2× diameter of the cells being electroporated;
- and
- an electrode positioned on either side of the flow channel and in direct contact with the fluid in the flow channel, the electrodes defining the narrowed portion of the flow channel;
- and wherein the electrodes apply one or more electric pulses to the cells in the fluid as they pass through the flow channel, thereby introducing the exogenous material into the cells in the fluid.
3 more elements
Dependencies are inferred from the claim text.