Selectively and fully cleavable fluorescent probes for sequential, itrative immunostaining

Abstract

The subject invention provides a selectively cleavable probe comprising an F(ab) fragment linked to one or more labels by a chemically cleavable disulfide bond. The subject invention also provides a process of immunostaining a sample comprising staining the sample with a selectively cleavable probe. The subject invention further provides a process of immunostaining a sample comprising (a) obtaining a probe comprising a primary antibody against a preselected target linked to biotin, (b) staining the sample with the probe of step (a), and (c) staining the sample with a selectively cleavable probe, wherein the F(ab) fragment is an anti-biotin F(ab) fragment.

Dependencies are inferred from the claim text.