A tentoxin synthesis gene, a method for producing tentoxin or dihydrotentoxin using the same, and a transformant comprising the same

A tentoxin synthesis-related gene, which encodes a protein having activity of nonribosomal peptide synthesis of dihydrotentoxin and containing the following modules in the order below from the N-terminal side:

• a first module having a first adenylation domain comprising the amino acid sequence of SEQ ID NO:
• 1 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 1, a first peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO:
• 2 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 2, and a first condensation domain comprising the amino acid sequence of SEQ ID NO:
• 3 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 3 in that order from the N-terminal side;
• a second module having a second adenylation domain comprising the amino acid sequence of SEQ ID NO:
• 4 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 4, a first N-methyltransferase domain comprising the amino acid sequence of SEQ ID NO:
• 5 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 5, a second peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO:
• 6 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 6, and a second condensation domain comprising the amino acid sequence of SEQ ID NO:
• 7 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 7 in that order from the N-terminal side;
• a third module having a third adenylation domain comprising the amino acid sequence of SEQ ID NO:
• 8 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 8, a third peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO:
• 9 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 9, and a third condensation domain comprising the amino acid sequence of SEQ ID NO:
• 10 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 10 in that order from the N-terminal side;
• and
• a fourth module having a fourth adenylation domain comprising the amino acid sequence of SEQ ID NO:
• 11 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 11, a second N-methyltransferase domain comprising the amino acid sequence of SEQ ID NO:
• 12 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 12, a fourth peptidyl carrier protein domain comprising the amino acid sequence of SEQ ID NO:
• 13 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 13, and a fourth condensation domain comprising the amino acid sequence of SEQ ID NO:
• 14 or an amino acid sequence having 70% or more identity with the amino acid sequence of SEQ ID NO:
• 14 in that order from the N-terminal side.