Methods for Purifying Clostridial Neurotoxin
AssigneeIpsen Biopharm Ltd.
InventorsGavin Stephen HACKETT, Shilpa PALAN, Dina Brady ANDERSON
Life of this patent
Estimating the term…
Abstract
A method for purifying a clostridial neurotoxin comprising contacting a cation exchange resin with a composition comprising a clostridial neurotoxin, wherein the contacting step is performed at at least pH 7.3, wherein the step of contacting a cation exchange resin with a composition comprising said clostridial neurotoxin occurs prior to conversion of the clostridial neurotoxin from a single chain form into a dichain form. Also provided are uses of a buffer having a pH value that is −1 pH unit or higher than the calculated pi of a clostridial neurotoxin, purification intermediates and clostridial neurotoxins obtainable by the invention, wherein the clostridial neurotoxin is in a single chain form.
Claims21
A method for purifying a clostridial neurotoxin, the method comprising contacting a cation exchange resin with a composition comprising a clostridial neurotoxin, wherein the contacting step is performed at a pH of 7.3 or higher and occurs prior to conversion of the clostridial neurotoxin from a single chain form into a di:
- chain form.
A purification intermediate comprising a clostridial neurotoxin associated with a cation exchange resin, wherein the purification intermediate has a pH of 7.3 or higher and the clostridial neurotoxin is in a single chain form.
A purification intermediate comprising a clostridial neurotoxin that has been separated from a cation exchange resin, wherein the purification intermediate has a pH of 7.3 or higher and the clostridial neurotoxin is in a single chain form.
13-14. (canceled)
18-19. (canceled)
(canceled)
Dependencies are inferred from the claim text.