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US 2019/0100553 A1Published application
Direct activity assays and compositions for nucleotide pool sanitizing enzymes
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsEric T. Kool, Debin Ji
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Abstract
Compositions and methods are provided for detecting activity of nucleotide pool repair enzymes. In the methods of the invention, a sample suspected of having nucleotide pool repair enzyme activity is combined with a detection compound provided herein, which detection compound comprises (i) a substrate for the enzyme of interest, (ii) a polyphosphate linker; and (iii) a detection moiety that is active when released by the enzyme of interest cleaving the linker.
Claims20
A detection compound having the structure:
- N-L-D I
- where N is a modified nucleoside substrate for a nucleotide pool repair enzyme;
- L is a polyphosphate linker of from about 3 to about 6 phosphates in length;
- and
- D is a detectable moiety selected from (i) adenosine and (ii) a fluorophore that is quenched when linked to the modified nucleoside, but detectable when released.
2 more elements
Dependencies are inferred from the claim text.