HUMAN iNKT CELL ACTIVATION USING GLYCOLIPIDS WITH ALTERED GLYCOSYL GROUPS

A method for augmenting an immunogenicity of an antigen in a subject in need thereof, comprising co-administering said antigen with an adjuvant composition comprising a GSLs compound of the general Formula (I):

• or a pharmaceutically acceptable salt thereof;
• wherein:
• R1 is —OH or halogen;
• R2 is —OH, or halogen;
• R3 is hydrogen;
• each instance of R4 and R5 is independently selected from the group consisting of hydrogen, halogen, optionally substituted alkyl, optionally substituted alkenyl, optionally substituted alkynyl, optionally substituted carbocyclyl, optionally substituted aryl, optionally substituted heterocyclyl, optionally substituted heteroaryl, optionally substituted alkoxy, an optionally substituted amino group, or optionally substituted acyl;
• n is an integer of 1 to 15, inclusive;
• m is an integer of 1 to 20, inclusive;
• wherein at least one of R1 or R2 is halogen.