Engineering gut commensal bacteria to express heterologous proteins in their outer membrane vesicles (omvs) for delivery to the gi-tract
AssigneesQuadram Institute Bioscience, UEA Enterprises Limited
InventorsREGIS GABRIEL STENTZ, SIMON RICHARD CARDING
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Abstract
This invention relates to the delivery of heterologous peptides or proteins such as therapeutic peptides, therapeutic proteins or antigens to mucosal sites using vesicles derived from the outer membrane of commensal bacteria, recombinant bacteria capable of producing such vesicles, and methods for the production of such vesicles. The invention further relates to an inducible expression system for use in recombinant bacteria.
Claims32
A recombinant gram negative commensal gut bacterium comprising an expression system for expression of a heterologous peptide or protein in an outer membrane vesicle (OMV).
A method for preparing an outer membrane vesicle (OMV) containing a heterologous peptide or protein, said method comprising:
- generating a recombinant gram negative commensal gut bacterium that expresses a heterologous peptide or protein;
- cultivating the bacterium under conditions that result in the expression of said heterologous peptide or protein and the production of OMV;
- and isolating OMV thereby containing said heterologous peptide or protein.
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Dependencies are inferred from the claim text.