Pd-l1 expressing hematopoietic stem cells and uses
AssigneeChildren's Medical Center Corporation
InventorPaolo Fiorina
Life of this patent
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Abstract
Embodiments disclosed here provide engineered modified hematopoietic stem cells (HSCs), artificially prostaglandin E2 (PGE2)-stimulated HSCs, compositions comprising these HSCs, methods of using these modified HSCs for treating autoimmune diseases and disorders and for suppressing the immune system. In particular, the engineered modified HSCs or PGE2-stimulated HSCs express the surface marker, programmed cell death-1 ligand 1 (PD-L1).
Claims31
(canceled)
A method of suppressing immune-mediated destruction of pancreatic islet β-cells in a subject with Type 1 diabetes (T1D), the method comprising:
- administering to a subject with T1D a composition comprising a population of modified PD-L1+-expressing hematopoietic stem cells (HSCs) in an amount effective to suppress immune-mediated destruction of pancreatic islet β-cells.
The method of claim 21, wherein the population of HSCs are ex vivo cultured before and/or after i) the introduction of the exogenous copy of a nucleic acid encoding PD-L1 or ii) contacting the HSCs with prostaglandin E2 (PGE2).
A method of treating the onset of pediatric Type 1 diabetes (T1D), the method comprising:
- administering to a pediatric subject with new onset T1D a therapeutically effective amount of a composition comprising a population of modified PD-L1+-expressing hematopoietic stem cells.
A method of treating the onset of adult Type 1 diabetes (T1D), the method comprising:
- administering to an adult subject with new onset T1D a therapeutically effective amount of a composition comprising a population of modified PD-L1+-expressing hematopoietic stem cells.
A method of delaying the onset of Type 1 diabetes (T1D) in a subject at risk of developing T1D, the method comprising:
- administering to the subject a composition comprising a population of modified PD-L1+ expressing hematopoietic stem cells (HSCs).
Dependencies are inferred from the claim text.