Calibration for oct-niraf multimodality probe

A multimodality system, comprising:

• a catheter configured to direct radiation from first and second modalities onto a plurality of locations of a sample, and to collect fluorescent light emitted in response to irradiating the sample with the radiation of the second modality;
• a detector configured to detect intensity of the fluorescent light received from the plurality of locations as a function of an angle α formed at each of the plurality of locations between the normal to the sample surface and the optical axis of the radiation incident on the sample;
• and
• a processor configured to calculate the angle α at each of the plurality of locations based on the radiation incident on the sample, and to adjust the intensity of the fluorescent light detected by the detector using a calibration factor g(α),
• wherein the calibration factor g(α) is function of the angle α calculated at two or more of the plurality of locations.

A method, comprising:

• irradiating a sample with radiation from first and second modalities via a catheter, and collecting via the catheter fluorescent light emitted in response to irradiating the sample with the radiation of the second modality;
• detecting intensity of the fluorescent light received at a detector from a plurality of locations of the sample as a function of an angle α formed at each of the plurality of locations between the normal to the sample surface and an optical axis of the radiation incident on the sample;
• calculating, using a processor, the angle α at each of the plurality of locations based on the radiation incident on the sample at each of the plurality of locations;
• and
• adjusting, using the processor, the intensity of the fluorescent light detected by the detector using a calibration factor g(α),
• wherein the calibration factor g(α) is function of the angle α calculated at two or more of the plurality of locations.

A method, comprising:

• transmitting radiation of at least three different wavelengths via a catheter having a proximal end and a distal end arranged along an axis, wherein the catheter directs a radiation of first wavelength and a radiation of second wavelength from its distal end thereof towards a sample along a line transverse to the axis, and collects at its distal end thereof a radiation of third wavelength from a plurality of locations of the sample;
• detecting, with a detector, intensity of the collected radiation of third wavelength as a function of an angle α formed at each of the plurality of locations between the normal to the sample surface and the optical axis of the radiation incident on the sample;
• calculating, using a processor, the angle α at each of the plurality of locations based on the radiation incident on the sample;
• and
• adjusting, using the processor, the intensity of the radiation of third wavelength detected by the detector using a calibration factor g(α),
• wherein the calibration factor g(α) is function of the angle α calculated at each of the plurality of locations, and
• wherein the processor adjusts the intensity of the radiation of third wavelength by multiplying the detected intensity by the calibration factor g(α) obtained at each of the plurality of locations.

A non-transitory computer-readable storage medium storing thereon computer executable code, which when executed by a processor, causes a computer to perform a method comprising:

• irradiating a sample with radiation from first and second modalities via a catheter, and collecting via the catheter fluorescent light emitted in response to irradiating the sample with the radiation of the second modality;
• detecting intensity of the fluorescent light received at a detector from a plurality of locations of the sample as a function of an angle α formed at each of the plurality of locations between the normal to the sample surface and an optical axis of the radiation incident on the sample;
• and
• calculating, using a processor, the angle α at each of the plurality of locations based on the radiation incident on the sample;
• and
• adjusting, using the processor, the intensity of the fluorescent light detected by the detector using a calibration factor g(α),
• wherein the calibration factor g(α) is function of the angle α calculated at two or more of the plurality of locations.