Taggable fluorescent probe for calcium ion detection
AssigneeThe University of Tokyo
InventorsKenzo HIROSE, Daisuke ASANUMA, Kohei MATSUI, Rieko TANAKA
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Abstract
A fluorescent probe for calcium ion detection that has an excellent photofading resistance and quick Ca2+ detection kinetics and can be localized at an arbitrary site in a cell is provided. The fluorescent probe contains a compound represented by the following general formula (I) or a salt thereof: A method for detecting intracellular calcium ions including (a) introducing the compound above or a salt thereof into a cell and (b) measuring the fluorescence emitted by the compound or a salt thereof in the cell is also provided.
Claims8
A compound represented by the following general formula (I) or a salt thereof:
- (wherein,
- R1 at each occurrence represents a hydrogen atom or the same or different monovalent substituent on the benzene ring;
- R2 and R3, each independently, represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms or a halogen atom;
- R4 and R5, each independently, represent a hydrogen atom, an alkyl group having 1 to 6 carbon atoms or a halogen atom;
- R6 represents an acetoxy group, an acetoxymethoxy group, or a hydroxyl group;
- X represents a linking group a between a benzene ring and U;
- U represents a group capable of capturing calcium ions after hydrolysis;
- Y represents a linking group b between a benzene ring and S;
- T, if present, represents a cross-linking group;
- S represents a label site;
- m is an integer of 1 to 3, and
- n is an integer of 1 to 3, with the proviso that m+n=4).
A compound represented by the following formula or a salt thereof.
Dependencies are inferred from the claim text.