Method of automatically modifying imaging range in biological sample microscopic imaging

A method for automatically altering an imaging area in microscopic imaging of a biological sample, comprising:

• (1) labeling an outline of the biological sample to differentiate the biological sample from a surrounding embedding medium;
• (2) setting an initial sample imaging area;
• imaging a sample surface layer using optical microscopy;
• wherein the initial sample imaging area is larger than an area to be imaged of the biological sample;
• (3) calculating an actual sample area using an outline identification algorithm based on an imaging result of the sample surface layer and setting the actual sample area as an imaging area of a next layer;
• (4) imaging a sample to be imaged of the next layer using optical microscopy based on the imaging area of the next layer set at step (3);
• wherein the imaging area of the next layer covers the area to be imaged of the biological sample, and there is no redundant imaging;
• (5) repeating steps (3) and (4) until data acquisition is completed.