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US 2019/0086301 A1Published application
Detection of cells in a liquid sample
AssigneeDelta Instruments B.V.
InventorsPierre Laurent EMOND, Nancy Gail PERLMUTTER, James Willis KREIDER
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Abstract
The invention relates to a method for counting cells, such as bacteria and/or somatic cells in liquid samples, such as in dairy products, preferably raw milk. Disclosed is a method comprising a combination of steps that apply dimeric nucleic acid dyes that normally do not penetrate cells (=cell-impermeant dyes), which are rendered cell-permeant by using the right combination of pH, buffer and temperature.
Claims19
1-33. (canceled)
A method of counting cells in a liquid sample, said method comprising the steps of:
- a) mixing a staining composition comprising a dimeric nucleic acid dye and a buffering agent with said sample;
- b) optionally sonicating the mixture of step a);
- c) incubating the mixture at a temperature from about 45° C. to about 95° C. for less than 10 minutes;
- d) optionally sonicating the incubated mixture of step c);
- and
- e) counting the cells that are stained with said dye within said mixture, or a part thereof, wherein the nucleic acid has the formula Q1-BRIDGE-Q2, wherein Q1 and Q2 are nucleic acid dye moieties and BRIDGE is connecting Q1 and Q2.
3 more elements
Dependencies are inferred from the claim text.