Sequencing library, and preparation and use thereof
AssigneeAgricultural Genomics Institute at Shenzhen China Academy of Agricultural Sciences
InventorsJue Ruan, Kaile Wang
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Abstract
The present invention discloses a sequencing library comprising a nucleotide sequence. The sequence comprises a linker sequence and two target sequences. Two ends of the linker sequence are respectively linked to the target sequences and the two target sequences are direct repeat sequences. The present invention further discloses preparation and use of the sequencing library. The present invention overcomes the high error rate problem of current DNA sequencing technologies, especially in a way of very low coverage bias, and can be used to detect low frequency mutations in different kinds of samples.
Claims18
A nucleotide sequence, comprising a linker and two target sequences, wherein two ends of the linker are respectively linked to the target sequences and the two target sequences are direct repeat sequences.
A nucleotide sequence, comprising a linker and two target sequences, wherein two ends of the linker are respectively linked to the target sequences and part of the regions of the two target sequences are direct repeat regions.
A sequencing kit, comprising an end repair, an A-tailing reagent, a DNA ligase, an adaptor sequence, a single-stranded circularization reagent, a nickase, and a strand displacement reagent.
Dependencies are inferred from the claim text.