Novel polyphosphate-dependent glucokinase and method for preparing glucose 6-phosphate by using same

A composition for the production of glucose 6-phosphate comprising a thermoresistant polyphosphate-dependent glucokinase derived from the genus Anaerolinea, glucose, and polyphosphate.

A method for producing glucose 6-phosphate from a composition comprising a thermoresistant polyphosphate-dependent glucokinase derived from the genus Anaerolinea, glucose, and polyphosphate.

A method for producing glucose 6-phosphate from a composition comprising a thermoresistant polyphosphate-dependent glucokinase derived from the genus Anaerolinea, liquefying and saccharifying enzymes, starch, and polyphosphate.

Escherichia coli BL21(DE3)/CJ_at_ppgk(KCCM11814P) transformed with a recombinant expression vector comprising the gene of SEQ ID NO. 1.

A method for producing a compound selected from D-glucose 1-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-myo-inositol 3-phosphate, D-myo-inositol, D-glucuronate, D-glucosamine 6-phosphate, D-glucosamine, N-acetyl-D-glucosamine 6-phosphate, N-acetyl-D-glucosamine, N-acetyl-D-mannosamine 6-phosphate, N-acetyl-D-mannosamine, N-acetylneuraminic acid (sialic acid), D-mannose 6-phosphate, D-mannose, D-tagatose 6-phosphate, D-tagatose, D-allulose 6-phosphate, D-allulose, D-glyceraldehyde 3-phosphate, and dihydroxyacetone phosphate, the method comprising reacting a thermoresistant polyphosphate-dependent glucokinase derived from the genus Anaerolinea, glucose or starch, polyphosphate, and an additional enzyme.