Maltooligosyl Trehalose Synthase Mutant and its Application
AssigneeJiangnan University
InventorsJing Wu, Lingqia Su, Kailin Yao
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of genetic engineering and enzyme engineering, and more particularly relates to a maltooligosyl trehalose synthase mutant and its application.The present invention provides a series of maltooligosyl trehalose synthase mutants with improved enzyme activity.
Claims8
A gene, wherein the gene encodes a maltooligosyl trehalose synthase mutant consisting of a mutation compared with the amino acid sequence of a wild-type maltooligosyl trehalose synthase from Sulfolobus acidocaldarius, the mutation being selected from a group consisting of:
- a substitution at position 81, a substitution at position 263, a substitution at position 432, a substitution at position 439, a substitution at position 583, a substitution at position 585, a substitution at position 586, a substitution at position 611, a substitution at position 615, and combinations thereof;
- wherein a parent amino acid sequence of the wild-type maltooligosyl trehalose synthase is set forth in SEQ ID NO:
- 1;
- wherein the maltooligosyl trehalose synthase mutant has enzymatic activity more than the wild-type maltooligosyl trehalose synthase.
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.