Methods for making high intensity sweeteners
AssigneeFirmenich SA
InventorsAndrew P. Patron, Chris Edano Noriega, Rama R. Manam, Justin Colquitt, Nathan Faber, Helge Zieler, Justin Stege
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Abstract
Provided herein include methods of making mogroside compounds, e.g., Compound 1, compositions (for example host cells) for making the mogroside compounds, and the mogroside compounds made by the methods disclosed herein, and compositions (for example, cell lysates) and recombinant cells comprising the mogroside compounds (e.g., Compound 1). Also provided herein are novel cucurbitadienol synthases and the use thereof.
Claims71
A method of producing Compound 1 having the structure of:
- the method comprising:
- contacting mogroside IIIE with a first enzyme capable of catalyzing a production of Compound 1 from mogroside IIIE.
-256. (canceled)
A cell lysate comprising Compound 1 having the structure:
A recombinant cell comprising a first gene encoding a first enzyme capable of catalyzing production of Compound 1 having the structure:
- from mogroside IIIE.
A method of producing Compound 1 having the structure of:
- the method comprising:
- contacting a first mogroside with one or more hydrolase to produce mogroside IIIE;
- and
- contacting the mogroside IIIE with an enzyme capable of catalyzing production of Compound 1 from mogroside IIIE.
A recombinant cell, comprising:
- a first gene encoding a hydrolase capable of hydrolyzing a first mogroside to produce Mogroside IIIE;
- and
- a second gene encoding an enzyme capable of catalyzing production of Compound 1 from Mogroside IIIE, wherein Compound 1 has the structure:
A fusion polypeptide having cucurbitadienol synthase activity, wherein the fusion polypeptide comprises a fusion domain fused to a cucurbitadienol synthase.
Dependencies are inferred from the claim text.