Production of gibberellins in recombinant hosts
AssigneeEvolva SA
InventorsEsben Halkjaer Hansen, Nina Nicoline Rasmussen, Michael Naesby, Jane Dannow Dyekjaer, Simon Carlsen, Adam Matthew Takos, Nicholas Ohler
Life of this patent
Estimating the term…
Abstract
The invention relates to recombinant microorganisms and methods for producing gibberellin compounds and gibberellin precursors.
Claims41
A recombinant host cell, comprising:
- (a) a recombinant gene encoding a first cytochrome P450 (P450) polypeptide;
- and/or
- (b) a recombinant gene encoding a 2-oxoglutarate-dependent dioxygenase (2-ODD) polypeptide and/or a second cytochrome P450 (P450) polypeptide;
- wherein the recombinant host cell is capable of producing a gibberellin precursor and/or a gibberellin compound.
A recombinant host cell, comprising:
- (a) a gene encoding a kaurenoic acid oxidase (KAO4) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:74;
- (b) a gene encoding a desaturase (DES) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:26;
- (c) a gene encoding a cytochrome P450 monooxygenase-2 (P450-2) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:80;
- and
- (d) a gene encoding a cytochrome P450 monooxygenase-3 (P450-3) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:186;
- wherein the recombinant host cell is capable of producing a gibberellin precursor and/or a gibberellin compound.
A recombinant host cell, comprising:
- (a) a gene encoding a kaurenoic acid oxidase (KAO4) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:74;
- (b) a gene encoding a gibberellin A20-oxidase (GA20ox) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:42;
- (c) a gene encoding a cytochrome P450 monooxygenase-3 (P450-3) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 186;
- and
- (d) a gene encoding a desaturase (DES) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:26;
- wherein the recombinant host cell is capable of producing a gibberellin precursor and/or a gibberellin compound.
A recombinant host cell, comprising a gene encoding a kaurenoic acid oxidase (KAO) polypeptide having at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:62, SEQ ID NO:60, or SEQ ID NO:152, at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:58 or SEQ ID NO:68, at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO:64, or at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:74;
- wherein the recombinant host cell is capable of producing gibberellin precursor and/or a gibberellin compound.
A recombinant host cell, comprising:
- (a) a gene encoding a kaurenoic acid oxidase (KAO11) polypeptide having at least 65% sequence identity to the amino acid sequence set forth in SEQ ID NO:64;
- and
- (b) a gene encoding a cytochrome P-450 BJ-1 (CYP112) polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:124;
- wherein the recombinant host cell is capable of producing a gibberellin precursor and/or a gibberellin compound.
A recombinant host cell, comprising:
- (a) a gene encoding a kaurenoic acid oxidase (KAO4) polypeptide having at least 50% sequence identity to the amino acid sequence set forth in SEQ ID NO:74;
- and
- (b) a gene encoding a cytochrome P-450 BJ-1 (CYP112) polypeptide having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO:124;
- wherein the recombinant host cell is capable of producing a gibberellin precursor and/or a gibberellin compound.
Dependencies are inferred from the claim text.