Systems and methods for forming a nanopore in a lipid bilayer
AssigneeGenia Technologies, Inc.
InventorsRoger J.A. Chen, Randy Davis
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Abstract
Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.
Claims14
A method of identifying a base of a nucleic acid molecule, comprising:
- recording a first electrical measurement indicative of the base of the molecule as a strand of the molecule moves through a nanopore under an applied electrical stimulus;
- recording a second electrical measurement indicative of the base of the molecule wherein the second electrical measurement is a repeat electrical measurement;
- and
- identifying the base based on the first electrical measurement wherein the repeat measurement is used to increase confidence in the identification.
A system for identifying a base of a nucleic acid molecule, comprising:
- a sensing circuit coupled to a nanopore;
- and
- a processor coupled to the sensing circuit, wherein the processor is programmed to:
- control the sensing circuit to record a first electrical measurement indicative of the base of the molecule as a strand of the molecule moves through a nanopore under an applied electrical stimulus;
- control the sensing circuit to record a second electrical measurement indicative of the base of the molecule wherein the second electrical measurement is a repeat electrical measurement;
- and
- identify the base based on the first electrical measurement wherein the repeat measurement is used to increase confidence in the identification.
Dependencies are inferred from the claim text.