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US 2019/0062818 A1Published application
Sensor, reagent, method for manufacturing probe molecule, and method for manufacturing polymer molecule
AssigneeKabushiki Kaisha Toshiba
InventorsHiroko Miki, Yoshiaki Sugizaki, Tatsuro Saito, Atsunobu Isobayashi
Life of this patent
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Abstract
According to one embodiment, a sensor includes an ionic liquid, a probe molecule, and a sensor element. The probe molecule selectively associates with a designated substance in the ionic liquid. The sensor element detects an association of the probe molecule with the designated substance.
Claims20
A sensor, comprising:
- an ionic liquid;
- a probe molecule selectively associating with a designated substance in the ionic liquid;
- and
- a sensor element detecting an association of the probe molecule with the designated substance.
1 more element
A sensor, comprising:
- an ionic liquid;
- a catalyst molecule promoting a designated chemical reaction in the ionic liquid;
- and
- a sensor element detecting at least one of a chemical substance or a charge, the at least one increasing or decreasing due to the chemical reaction.
1 more element
A reagent, comprising:
- an ionic liquid;
- a first probe molecule selectively associating with a designated substance in the ionic liquid;
- a first fluorescent dye;
- and
- at least one of a second fluorescent dye or a quencher,
- due to the first probe molecule associating with the designated substance, a distance between the first fluorescent dye and the at least one of the second fluorescent dye or the quencher changes, and a fluorescence intensity due to a resonance energy transfer changes.
3 more elements
A method for manufacturing a probe molecule, comprising:
- mixing a plurality of nucleic acids having different base sequences with a designated substance in an ionic liquid;
- and
- obtaining an aptamer associating in the ionic liquid with the designated substance by performing amplification in an aqueous solution after selecting and recovering the nucleic acid having associated with the designated substance.
A method for manufacturing a probe molecule, comprising:
- using a ribosome to synthesize a polypeptide having different amino acid sequences from a plurality of mRNA (messenger RNA) having different base sequences;
- mixing, with a designated substance into an ionic liquid, a complex in which the mRNA, the ribosome, and the polypeptide are not separated;
- and
- obtaining a peptide aptamer associating with the designated substance by selecting and recovering the complex having associated at a polypeptide site with the designated substance, subsequently extracting mRNA and performing reverse transcription into DNA, performing amplification of the DNA using PCR (Polymerase Chain Reaction) or LAMP (Loop-Mediated Isothermal Amplification), performing transcription into mRNA, subsequently repeating processes of synthesizing a polypeptide, mixing with the designated substance in an ionic liquid, selecting and recovering an associated substance, performing reverse transcription, performing amplification, and performing transcription, and subsequently synthesizing a polypeptide from the mRNA by using a ribosome.
1 more element
A method for manufacturing a probe molecule, comprising:
- immobilizing a plurality of probe molecules in an array configuration on a substrate, the plurality of probe molecules including at least one type selected from the group consisting of a plurality of nucleic acids having different base sequences, a plurality of polypeptides having different amino acid sequences, and a plurality of antibodies having different paratopes;
- and
- selecting a designated probe molecule by immersing, in an ionic liquid with a designated substance, the plurality of probe molecules immobilized on the substrate and by verifying an association ability of the plurality of probe molecules and the designated substance in the ionic liquid.
A method for manufacturing a polymer molecule associating with a designated substance, comprising:
- mixing the designated substance and a monomer molecule in an ionic liquid, the monomer molecule having a site associating with the designated substance in the ionic liquid and a second reaction site;
- polymerizing a polymer precursor molecule on an associated substance of the designated substance and the monomer molecule after the monomer molecule has associated with the designated substance, the polymer precursor molecule having a site binding to the second reaction site of the monomer molecule, the polymer precursor molecule becoming a polymer by polymerization;
- and
- subsequently removing the designated substance.
1 more element
Dependencies are inferred from the claim text.