Methods and materials for the production of monomers for nylon-4/polyester production
AssigneeInvista North America S.A R.L.
InventorsAlex Van Eck CONRADIE, Adriana Leonora BOTES
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Abstract
This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.
Claims39
A non-naturally occurring method of producing 1,4-butanediol, comprising enzymatically converting 4-hydroxybutyrate to 1,4 butanediol using a carboxylate reductase and an alcohol dehydrogenase.
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10-13. (canceled)
A method of producing putrescine, comprising:
- enzymatically converting 4-hydroxybutyrate to putrescine using a carboxylate reductase, an alcohol dehydrogenase, and at least one ω-transaminase;
- enzymatically converting 4-aminobutyrate to putrescine using a carboxylate reductase and a ω-transaminase;
- or
- enzymatically converting 1.4 butanediol to putrescine using at least one alcohol dehydrogenase and at least one ω-transaminase.
15-19. (canceled)
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A recombinant host comprising:
- at least one exogenous nucleic acid encoding (i) a glutamate decarboxylase, (ii) an alcohol dehydrogenase selected from a 4-hydroxybutyrate dehydrogenase and a 5-hydroxyvalerate dehydrogenase, and (iii) a first exogenous ω-transaminase;
- or
- at least one exogenous nucleic acid encoding (i) a 2-oxoglutarate decarboxylase and (ii) an alcohol dehydrogenase selected from a 4-hydroxybutyrate dehydrogenase and a 5-hydroxyvalerate dehydrogenase,
- wherein the recombinant host produces 4-hydroxybutyrate.
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39-50. (canceled)
A composition comprising:
- 4-hydroxybutyrate;
- a bio-derived 1,4 butanediol or a bio-derived putrescine;
- and
- a carboxylate reductase, an alcohol dehydrogenase, and optionally at least one ω-transaminase.
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55-58. (canceled)
A nucleic acid construct or expression vector comprising:
- (a) a polynucleotide encoding a polypeptide having the activity of a glutamate decarboxylase, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having the activity of a glutamate decarboxylase is selected from polypeptides having at least 70% sequence identity to the polypeptide of SEQ ID NO:
- 14 or SEQ ID NO:
- 19;
- (b) a polynucleotide encoding a polypeptide having the activity of a carboxylate reductase, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having the activity of a carboxylate reductase is selected from polypeptides having at least 70% sequence identity to the polypeptide of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, or SEQ ID NO:
- 18;
- (c) a polynucleotide encoding a polypeptide having the activity of a ω-transaminase, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having the activity of a ω-transaminase is selected from polypeptides having at least 70% sequence identity to the polypeptide of SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or SEQ ID NO:
- 11;
- (d) a polynucleotide encoding a polypeptide having the activity of a phosphopantetheinyl transferase, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having the activity of a phosphopantetheinyl transferase is selected from polypeptides having at least 70% sequence identity to the polypeptide of SEQ ID NO:
- 12 or 13;
- or
- (e) a polynucleotide encoding a polypeptide having the activity of a decarboxylase, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide and wherein the polypeptide having the activity of a decarboxylase is selected from polypeptides having at least 70% sequence identity to the polypeptide of SEQ ID NO:
- 15, 16, or 17.
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Dependencies are inferred from the claim text.