Reoviridae vaccine
AssigneeLondon School of Hygiene and Tropical Medicine
InventorPolly ROY
Life of this patent
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Abstract
The invention relates to an isolated African horse sickness virus (AHSV) ssRNA comprising a plurality of mutations; a complementary cell for replication of a vaccinal viral strain from said ssRNA; a vaccinal viral strain derived from said ssRNA; use of said vaccinal viral strain and/or isolated ssRNA in the vaccination of an animal against an infection by AHSV; a method of vaccination comprising same; and a pharmaceutical composition comprising said vaccinal viral strain and/or said isolated ssRNA.
Claims26
An isolated viral ssRNA derived from the genome of African Horse Sickness Virus (AHSV) wherein the ssRNA comprises at least a part of S9 segment of said virus and having therein a plurality of mutations in S9 segment of said virus and further wherein each mutation, at least after being introduced into said ssRNA, provides or encodes a stop codon.
-19. (canceled)
A kit comprising:
- an isolated viral ssRNA derived from the genome of African Horse Sickness Virus (AHSV), wherein the ssRNA comprises at least a part of S9 segment of said virus and having therein a plurality of mutations in S9 segment of said virus and further wherein each mutation, at least after being introduced into said ssRNA, provides or encodes a stop codon;
- and
- a cell expressing at least one essential gene of the S9 segment of AHSV and that complements said essential gene mutated in the ssRNA, which thereby enables the replication of a vaccinal viral strain in the cell when infected with the ssRNA.
A method for generating defective AHSV virus of a given serotype comprising:
- a) introducing a plurality of mutations in S9 segment of said virus wherein each mutation, at least after being introduced into said ssRNA, provides or encodes a stop codon;
- and
- b) reassorting or exchanging the following selected segments S2+S6;
- S2+S6+S7;
- S2+S6+S7+S3;
- or S2+S6+S7+S3+S10 to generate the following serotypes def-AHSV8 and def-AHSV9 serotypes;
- def-AHSV3 and def-AHSV4;
- def-AHSV5, def-AHSV6 and def-AHSV7;
- and def-AHSV2 respectively.
A method for generating defective AHSV virus of a given serotype comprising:
- a) introducing a plurality of mutations in S9 segment of said virus wherein each mutation, at least after being introduced into said ssRNA, provides or encodes a stop codon;
- and
- b) reassorting or exchanging the following selected segments to generate the following serotypes:
- the reassortment of two segments (S2+S6) encoding outer shell proteins VP2 and VP5 to generate def-AHSV8 and def-AHSV9 serotypes;
- and/or
- the reassortment of the two segments (S2+S6) encoding outer shell proteins VP2 and VP5 and the segment (S7) encoding middle-core protein VP7 to generate def-AHSV3 and def-AHSV4;
- and/or
- the reassortment of the two segments (S2+S6) encoding outer shell proteins VP2 and VP5 and the segment (S7) encoding middle-core protein VP7 and the segment (S3) encoding middle-core protein VP3 to generate def-AHSV5, def-AHSV6 and def-AHSV7;
- and/or
- the reassortment of the two segments (S2+S6) encoding outer shell proteins VP2 and VP5 and the segment (S7) encoding middle-core protein VP7 and the segment (S3) encoding middle-core protein VP3 and the segment (S10) encoding NS3/NS3A to generate def-AHSV2.
Dependencies are inferred from the claim text.