Diagnostic Method
AssigneeCytosystems Limited
InventorsDavid Galloway, Nick Coleman
Life of this patent
Estimating the term…
Abstract
The invention provides a method of detecting a subject suffering from, or at risk of suffering from, bladder cancer the method comprising i) providing a body fluid sample isolated from a subject; ii) isolating cells from said sample to provide a cell sample; iii) contacting the sample with a specific binding member capable of binding to a minichromosome maintenance (MCM) polypeptide(s); iv) determining the binding of said specific binding member to the cell sample; v) counting those cells in said cell sample which bound to said specific binding member to provide a cell count; vi) determining, based on the cell count, whether the subject has, or is at risk of having, bladder cancer.
Claims15
A method of detecting a subject suffering from, or at risk of suffering from, bladder cancer the method comprising
- i) providing a urine sample isolated from a subject;
- ii) isolating cells from said sample to provide a cell sample;
- iii) contacting the sample with a specific binding member capable of binding to a minichromosome maintenance (MCM) polypeptide(s);
- iv) determining the binding of said specific binding member to the cell sample;
- v) counting those cells in said cell sample which bound to said specific binding member to provide a cell count;
- and,
- vi) determining, based on the cell count, whether the subject has, or is at risk of having, bladder cancer;
- wherein said determining step (vi) is based upon a measurement of MCM labelled cells relative to a threshold number, wherein said measurement above or equal to said threshold is indicative of bladder cancer or a risk of bladder cancer, and wherein the threshold number is about 30 to 50 cells or about 50 to 200 cells when the subject has previous evidence of bladder cancer.
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5-18. (canceled)
A method of detecting bladder cancer in a subject who has a previous occurrence of bladder cancer or has relapsed, the method comprising:
- i) providing a urine sample isolated from a subject;
- ii) isolating cells from said sample to provide a cell sample;
- iii) contacting the sample with a specific binding member capable of binding to a minichromosome maintenance (MCM) polypeptide(s);
- iv) determining the binding of said specific binding member to the cell sample;
- v) counting those cells in said cell sample which bound to said specific binding member to provide a cell count;
- and,
- vi) determining, based on the cell count, whether the subject has, or is at risk of having, a recurrence of bladder cancer;
- wherein said determining step (vi) is based upon a measurement of MCM bound cells relative to a threshold number, wherein said measurement above or equal to said threshold is indicative of bladder cancer or a risk of recurrence of bladder cancer and wherein the threshold number is about 50 to 200 cells.
20-26. (canceled)
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Dependencies are inferred from the claim text.