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US 2019/0056398 A1Published application
Identification and isolation of antibodies from white blood cells
OwnerFeng Wang-Johanning, Gary L. Johanning +4inventors
InventorsFeng Wang-Johanning, Gary L. Johanning, Nathan Collins, Xiaohe Liu, Zheng Ao, Lidia Sambucetti
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Abstract
Embodiments in accordance with the present disclosure include apparatuses, devices, and methods. For example, a method is directed to method exposing immobilized white blood cells from a blood sample to an antigen. And, scanning the immobilized white blood cells and, therefrom, identify and isolate white blood cells from among the immobilized white blood cells that produce an antibody in response to the exposure to the antigen.
Claims20
A method comprising:
- exposing immobilized white blood cells from a blood sample to an antigen;
- and
- scanning the immobilized white blood cells and, therefrom, identify and isolate white blood cells from among the immobilized white blood cells that produce an antibody (Ab) in response to the exposure to the antigen.
A method comprising:
- causing a substrate coated with an antigen to contact a multiple-well array and thereby exposing a plurality of white blood cells to the antigen, each well of the multiple-well array including an individual white blood cell among the plurality of white blood cells;
- causing each well of the multiple-well array to include an individual target cell;
- and
- scanning the substrate and the wells of the multiple-well array and, therefrom, assessing an efficacy and cell function of the plurality of white blood cells.
1 more element
A method comprising:
- on a substrate, immobilizing white blood cells from a blood sample;
- exposing the immobilized white blood cells to an antigen;
- identifying white blood cells from among the immobilized white blood cells that produce an antibody (Ab) in response to the exposure to the antigen by scanning the substrate using an optic scanner;
- isolating the identified white blood cells from the substrate;
- exposing a plurality of white blood cells, including the isolated white blood cells, to the antigen by causing an additional substrate coated with the antigen to contact a multiple-well array, each well of the multiple-well array including an individual white blood cell among the plurality of white blood cells;
- exposing the plurality of white blood cells to target cells by causing each well of the multiple-well array to include an individual target cell;
- and
- assessing an efficacy and cell function of the plurality of white blood cells by scanning the additional substrate and the wells of the multiple-well array using the optic scanner and a fluorescent microscope.
5 more elements
An apparatus comprising:
- an optic scanner, a light source, and imaging circuitry, the optic scanner configured to scan white blood cells from a blood sample and, therefrom, identify white blood cells that produce an antibody responsive to exposure to an antigen;
- a fluorescent microscope configured to scan the white blood cells and, therefrom, verify the produced antibodies and assess an efficacy of the produced antibodies;
- and
- cell picking circuitry configured to isolate antibodies responsive to the verification and the assessment.
1 more element
Dependencies are inferred from the claim text.