Systems and compositions for diagnosing pathogenic fungal infection and methods of using the same
AssigneeThe Regents of the University of California
InventorsClarissa Nobile, Michael Winter, Charles Craik, Alexander Johnson, Anthony O'Donoghue
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Abstract
The present disclosure relates generally to detection of contamination of a sample or diagnosis of subject based upon detection or quantification of amino acid sequences in a sample, specifically to the identification and use of molecular biomarkers for Candida albicans biofilm infections.
Claims24
A method of diagnosing a subject with a pathogenic fungal infection comprising:
- detecting the presence, absence and/or quantity of at least one aminopeptidase, secreted aspartyl protease (Sap), subtilisin-family protease or functional fragment thereof in a sample.
(canceled)
-10. (canceled)
A method of diagnosing a pathogenic infection of C. albicans in a subject comprising:
- (a) contacting a plurality of probes specific for Sap5 and/or Sap6, or a functional fragment thereof with a sample;
- (b) quantifying the amount of Sap5 and/or Sap6, or a functional fragment thereof in the sample;
- (c) calculating one or more scores based upon the presence, absence, or quantity of Sap5 and/or Sap6, or a functional fragment thereof;
- and
- (d) correlating the one or more scores to the presence, absence, or quantity of Sap5 and/or Sap6, or a functional fragment thereof, such that if the amount of Sap5 and/or Sap6, or functional fragment thereof is greater than the quantity of Sap5 and/or Sap6, or a functional fragment thereof, in a control sample, the correlating step comprises diagnosing a subject with a pathogenic infection of C. albicans.
-23. (canceled)
A method for characterizing the stage of development or pathology of a fungal infection, comprising:
- (a) contacting a plurality of probes specific for Sap5 and/or Sap6, or a functional fragment thereof with a sample;
- (b) quantifying the amount of Sap5 and/or Sap6, or a functional fragment thereof in the sample;
- (c) calculating one or more normalized scores based upon the presence, absence, or quantity of Sap5 and/or Sap6 or a functional fragment thereof;
- and
- (d) correlating the one or more scores to the presence, absence, or quantity of Sap5 and/or Sap6, or a functional fragment thereof, such that if the amount Sap5 and/or Sap6, or functional fragment thereof is greater than the quantity of Sap5 and/or Sap6, or a functional fragment thereof in a control sample, the correlating step comprises characterizing the sample as comprising a fungal infection as pathogenic.
(canceled)
A method of detecting the presence of a pathogenic fungus in a sample comprising:
- (a) contacting a plurality of probes specific for Sap5 and/or Sap6, or a functional fragment thereof with a sample;
- (b) quantifying the amount of Sap5 and/or Sap6, or a functional fragment thereof in the sample;
- (c) calculating one or more normalized scores based upon the presence, absence, or quantity of Sap5 and/or Sap6 or a functional fragment thereof;
- and
- (d) correlating the one or more scores to the presence, absence, or quantity of Sap5 and/or Sap6, or a functional fragment thereof, such that if the amount Sap5 and/or Sap6, or functional fragment thereof is greater than the quantity of Sap5 and/or Sap6, or a functional fragment thereof in a control sample, the correlating step comprises characterizing the sample as comprising a fungal infection as pathogenic.
(canceled)
Dependencies are inferred from the claim text.