Protein tag that binds to substances having a crystal structure, and uses thereof
AssigneeHiroshima University
InventorsAkio KURODA, Takenori ISHIDA
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Abstract
In order to provide a protein tag and use thereof, the present invention uses a protein tag that binds to a substance having a crystal structure and that includes a binding domain whose amino acid sequence is a mutated version of an amino acid sequence of an ice-binding domain derived from an antifreeze protein.
Claims8
A protein tag which binds to a substance having a crystal structure,
- the protein tag comprising a binding domain whose amino acid sequence is a mutated version of an amino acid sequence of an ice-binding domain derived from an antifreeze protein.
A method for screening a protein tag which binds to a target substance having a crystal structure, the method comprising the steps of:
- bringing a phage and a target substance having a crystal structure into contact with each other, the phage displaying, by a phage display method, a protein tag that includes a binding domain whose amino acid sequence is a mutated version of an amino acid sequence of an ice-binding domain derived from an antifreeze protein;
- and
- recovering the phage bound to the target substance having a crystal structure.
A kit for screening a protein tag which binds to a target substance having a crystal structure, the kit comprising a phage vector or a phagemid vector each of which contains a gene coding for a protein tag, the protein tag including a binding domain whose amino acid sequence is a mutated version of an amino acid sequence of an ice-binding domain derived from an antifreeze protein.
A method for producing a protein tag which binds to a target substance having a crystal structure, the method comprising the steps of:
- bringing a phage and a target substance having a crystal structure into contact with each other, the phage displaying, by a phage display method, a protein tag that includes a binding domain whose amino acid sequence is a mutated version of an amino acid sequence of an ice-binding domain derived from an antifreeze protein;
- and
- recovering the phage bound to the target substance having a crystal structure.
Dependencies are inferred from the claim text.