System and method for determining the presence or absence of adsorbed biomolecules or biomolecular structures on a surface
OwnerAykutlu Dana, Sencer Ayas +1inventors
InventorsAykutlu Dana, Sencer Ayas, Ayse Begum Tekinay
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Abstract
An optical sensing method with optical excitation in the ultraviolet (UV) range (200-400 nm wavelength) where biomolecules, which have inherent absorption lines in the UV range, adsorb on a plasmonic surface exhibiting coincident optical resonances in the UV, and the amplitude of the reflected/scattered/transmitted optical wave, which is strongly changed due to the presence of biomacromolecules or biomolecular structures, are measured or imaged to infer the presence and optical properties of the adsorbed biomolecular structures.
Claims27
A method for determining the presence or absence of adsorbed biomolecules or biomolecular structures on a surface, the method comprises the steps of:
- (A) providing a plasmonic substrate, wherein a receiving surface of the plasmonic substrate exhibits plasmonic resonances at an ultraviolet (UV) wavelength range;
- (B) placing a test solution in contact with the receiving surface;
- (C) exciting the plasmonic substrate with an incident UV light wave with transverse magnetic polarization as the incident UV light wave illuminates the testing solution and the receiving surface;
- (D) measuring a surface plasmon resonance (SPR) profile for a resultant portion of the incident UV light wave, wherein the resultant portion is either reflected, scattered, or transmitted from the incident UV light wave;
- and
- (E) detecting a biomolecular analyte within the test solution, if the SPR profile for the resultant portion indicates overlapping molecular and plasmonic absorption spectra.
The method for determining the presence or absence of adsorbed biomolecules or biomolecular structures on a surface, the method as claimed in claim 10, wherein the rectangularly-profiled surface relief grating is shaped with a duty cycle ranging between 1% and 50% and is shaped with a corrugation depth ranging between 10 nm and 100 nm.
Dependencies are inferred from the claim text.