Improved assay

Abstract

The application describes a method of quantifying a multimeric analyte in a sample comprising: (i) treating the multimeric analyte with a separating agent selected to convert at least a portion of the multimeric analyte into monomeric analyte; (ii) binding the analyte to an analyte-specific binding agent; (iii) comparing the amount of binding of the analyte to the analyte-specific binding agent to a calibration curve, wherein the calibration curve is obtained (i) by binding the analyte-specific binding agent to one or more predetermined amounts of separating agent treated analyte, prior to contacting with analyte-specific binding agent or (ii) by binding the analyte-specific binding agent to a predetermined amount of substantially monomeric analyte; and (iv) determining an amount of the multimeric analyte in the sample.

Dependencies are inferred from the claim text.