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US 2019/0056299 A1Published application
Enzymatic sample purification
AssigneeHewlett-Packard Development Company, L.P.
InventorsViktor SHKOLNIKOV, Anita ROGACS
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Abstract
An enzymatic sample purifier comprises a reaction chamber containing an enzyme, a product chamber and a separator. The reaction chamber has a port to receive a sample comprising a protein degradation product. The separator retains polypeptides resulting from reaction of the protein degradation product with the enzyme in the reaction chamber and facilitates transport of purified remaining portions of the sample comprising a target analyte to the product chamber.
Claims15
An apparatus comprising:
- a reaction chamber containing an enzyme, the reaction chamber having a port to receive a sample comprising a protein degradation product;
- a product chamber;
- and
- a separator to retain polypeptides resulting from reaction of the protein degradation product with the enzyme in the reaction chamber and to transport purified remaining portions of the sample comprising a target analyte to the product chamber.
1 more element
A method comprising:
- depositing a sample comprising a target analyte and a protein degradation product into a reaction chamber containing an enzyme, to form a solution in the reaction chamber;
- reacting the protein degradation product with the enzyme to produce a polypeptide;
- and
- separating the polypeptide and the target analyte.
1 more element
An enzymatic sample purifier comprising:
- a reaction chamber containing an enzyme, the reaction chamber having a port to receive a sample comprising a protein degradation product;
- and
- a porous matrix within the reaction chamber, the porous matrix to retain polypeptides resulting from reaction of the protein degradation product with the enzyme in the reaction chamber.
Dependencies are inferred from the claim text.