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US 2019/0055607 A1Published application
Methods and compositions for determining specific tcr and bcr chain pairings
AssigneeGirihlet Inc.
InventorAnitha JAYAPRAKASH
Life of this patent
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Abstract
Provided are methods for determining TCR α/γ and TCR β/δ chain pairings in T cell(s) of interest within a population of T cells, comprising a combination of selecting for the T cell(s) of interest based on the TCR α/δ or TCR β/δ chain followed by sequencing of the counterpart TCR β/δ or TCR α/γ chain, respectively.
Claims18
A method for identifying T cell receptor chain pairings in T cell(s) of interest within a population of T cells, comprising:
- a) contacting said population of T cells with a plurality of probes directed to either the TCR α/γ chain or the TCR β/δ chain in the T cell(s) of interest, wherein said probes target at least a portion of the variable domain of the individual receptor chain;
- b) sorting said population of T cells based on binding of said plurality of probes to select for said T cell(s) of interest;
- and
- c) sequencing the counterpart TCR β/δ chain or TCR α/γ chain, respectively, in the sorted T cell(s) of interest.
1 more element
A method for identifying heavy and light chain pairings in a B cell(s) of interest within a population of B cells, comprising:
- a) contacting said population of B cells with a plurality of probes directed to either the heavy chain or light chain in the B cell(s) of interest, wherein said probes target at least a portion of the variable domain of the individual chain;
- b) sorting said population of B cells based on binding of said plurality of probes to select for said B cell(s) of interest;
- and
- c) sequencing the counterpart light chain or heavy chain, respectively, in the sorted B cell(s).
1 more element
Dependencies are inferred from the claim text.