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US 2019/0055591 A1Published application
Methods for fast nucleic acid amplification
AssigneeUniversity of Utah Research Foundation
InventorsCarl T. Wittwer, Jared Steven Farrar
Life of this patent
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Abstract
Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.
Claims19
A kit for performing PCR on a target nucleic acid, the kit comprising:
- dNTPs,
- a polymerase provided at a concentration of at least 0.5 μM, and
- a pair of primers configured for amplifying the target nucleic acid, wherein each primer of the pair of primers are provided at a concentration of at least 2 μM.
A kit for performing PCR on a target nucleic acid, the kit comprising:
- dNTPs,
- a thermostable polymerase, and
- primers configured for amplification of the target nucleic acid sequence to the biological sample,
- wherein the polymerase to primer ratio is (0.03 to 0.4 polymerase):(total primer concentration) and the polymerase has a concentration of at least 0.5 μM.
1 more element
A reaction mixture for performing PCR on a target eukaryotic DNA, the reaction mixture comprising:
- dNTPs,
- a polymerase provided at a concentration of at least 0.5 μM,
- a pair of primers configured for amplifying the target eukaryotic DNA, wherein each primer of the pair of primers are provided at a concentration of at least 2 μM, and
- a sample of eukaryotic genomic DNA.
1 more element
Dependencies are inferred from the claim text.