Optimized host cells for the production of glutathione
AssigneeLallemand SAS
InventorsCaroline Wilde, Corinne Cluis
Life of this patent
Estimating the term…
Abstract
The disclosure concerns a genetically modified host cell for the production and accumulation of glutathione (GSH). The genetically modified host cell can allow the expression of a mutated Cys4p whose activity is increased. In addition or alternatively, the genetically modified host cell can express a mutated Yap1p whose translocation from the nucleus to the cytoplasm is reduced. Furthermore, in addition or alternatively, the genetically modified host cell can express an heterologous threonine aldolase (Gly1p).
Claims26
A process of making glutathione, said process comprising fermenting a substrate with a genetically modified host cell to obtain a fermented mixture comprising glutathione, wherein the genetically modified host cell comprises at least one of:
- a first heterologous nucleic acid molecule coding for a mutated cystathionine beta-synthase protein (Cys4p) having an increased biological activity when compared to a wild-type Cys4p;
- a second heterologous nucleic acid molecule coding for a mutated Yap1p having a reduced ability of being translocated from nucleus to cytoplasm of the genetically modified host cell when compared to a wild-type Yap1p;
- and
- a third heterologous nucleic acid molecule coding for a threonine aldolase protein (Gly1p).
15-18. (canceled)
20-21. (canceled)
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28-29. (canceled)
A genetically modified host cell comprising at least two of:
- a first heterologous nucleic acid molecule encoding a mutated cystathionine beta-synthase protein (Cys4p) having an increased biological activity when compared to a wild-type Cys4p;
- a second heterologous nucleic acid molecule encoding a mutated Yap1p having a reduced ability of being translocated from nucleus to cytoplasm of the genetically modified host cell when compared to a wild-type Yap1p;
- and
- a third heterologous nucleic acid molecule coding for a threonine aldolase protein (Gly1p).
31-49. (canceled)
Dependencies are inferred from the claim text.