Crispr mediated recording of cellular events

A recombinant cell comprising one or more nucleic acid molecules encoding a CRISPR-Cas system comprising a guide RNA that targets a selected recorder DNA sequence and a Cas protein capable of modifying a targeted genomic locus, whereby at least one of said nucleic acid molecules encoding at least one of said guide RNA or Cas protein is operably connected with a regulatory element comprising a promoter that is activated in parallel to a cellular event or as a consequence of a cellular event.

A method of recording a cellular event in a cell and detecting the occurrence of the cellular event at a point of assay after the event has occurred comprising:

• (a) providing a cell comprising a recombinase operably connected in the cell with a regulatory element comprising a promoter that is activated in parallel to the cellular event or as a consequence of the cellular event, and a recorder DNA sequence comprising recombination sites for said recombinase, whereby expression of the recombinase is driven by the promoter;
• and
• (b) detecting the occurrence of the cellular event based on detection of the recombination of said recorder DNA sequence.

A recombinant cell comprising a nucleic acid molecule encoding a recombinase operably connected with a regulatory element comprising a promoter that is activated in parallel to a cellular event or as a consequence of a cellular event and a recorder DNA sequence comprising recombination sites for said recombinase.