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US 2019/0055580 A1Published application
Hybrid promoter and uses thereof
AssigneeJust Biotherapeutics, Inc.
InventorsJeffrey T. McGrew, Pauline S. Smitdt, E-Ching Ong
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Abstract
A hybrid promoter for recombinant expression of proteins of interest is disclosed that combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Also disclosed are an expression cassette containing the hybrid promoter and a recombinant expression vector containing the expression cassette. A mammalian host cell, which comprises the recombinant expression vector is also disclosed, as is a method of producing a protein of interest that employs the mammalian host cell, optionally involving tetracycline-inducible expression of the protein.
Claims24
A hybrid promoter, comprising:
- (i) a mCMV enhancer sequence, comprising a mCMV enhancer element (mCMV-E) and a CMV promoter (CMV-P) sequence at its 3′ end, operably linked 5′ to a rat EF-1alpha intron sequence;
- (ii) an intervening first leader sequence operably linked, 3′ to the CMV promoter sequence of the mCMV enhancer sequence, and 5′ to the rat EF-1alpha intron sequence;
- and
- (iii) a second leader sequence operably linked 3′ to the rat EF-1alpha intron sequence.
1 more element
Dependencies are inferred from the claim text.