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AssigneesBoard of Trustees of the University of Arkansas, University of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsEllen M. Brune, Robert R. Beitle, Jr., Mohammad M. Ataai, Patrick R. Bartlow, Ralph L. Henry
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Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.
An isolated E. coli host cell for expression of a target host cell peptide, polypeptide, or protein, or a target recombinant peptide, polypeptide, or protein, wherein the genome of said isolated E. coli host cell is:
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