Nucleic acid constructs for producing retroviral vectors
AssigneeUCL Business PLC
InventorsMartin Pulé, Leila Mekkaoui
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Abstract
The present invention relates to a nucleic acid construct comprising: (i) a first nucleic acid sequence which either comprises a retroviral transfer vector or which encodes a retroviral protein; and (ii) a second nucleic acid sequence which encodes a detectable marker which is a cell surface protein comprising an extracellular domain and a membrane targeting domain.
Claims34
A nucleic acid construct comprising:
- (i) a first nucleic acid sequence which either comprises a retroviral transfer vector or which encodes a retroviral protein;
- and
- (ii) a second nucleic acid sequence which encodes a detectable marker which is a cell surface protein comprising an extracellular domain and a membrane targeting domain.
A nucleic acid construct according to any preceding claim wherein the first nucleic acid sequence encodes a retroviral protein selected from gag-pol, env or rev.
A nucleic acid construct according to any preceding claim wherein the cell surface protein has less than 200 amino acids.
A nucleic acid construct according to any preceding claim wherein the membrane targeting domain of the cell surface protein comprises (i) a transmembrane domain;
- or (ii) a GPI anchor.
A nucleic acid construct according to any preceding claim wherein the membrane targeting domain comprises a CD8 stalk or a part thereof.
A nucleic acid construct according to any preceding claim wherein the extracellular domain comprises HA, V5, RQR8 or MYC.
A nucleic acid construct according to any preceding claim wherein the cell surface protein comprises an amino acid sequence as shown as SEQ ID NO:
- 21-26.
A nucleic acid construct according to any preceding claim which comprises transposon sequences flanking the first and second nucleic acid sequence sequences.
A kit comprising a plurality of nucleic acid constructs according to any preceding claim.
Dependencies are inferred from the claim text.