Prevention, diagnosis and treatment of cancer overexpressing gpr160
AssigneeShanghai Institute of Material Medica, Chinese Academy of Sciences
InventorsCaihong Zhou, Xinchuan Dai, Mingwei Wang
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Abstract
Provided are a dsRNA construct of an orphan G-protein-coupled receptor GPR160 gene related to prostate cancer and the use thereof, wherein the dsRNA construct of the GPR160 gene and a composition thereof can prevent or treat prostate cancer.
Claims19
A dsRNA construct, wherein the dsRNA construct is double-stranded and the forward or reverse chain thereof has the structure shown in Formula I:
- SeqForward-X-SeqReverse Forward Formula I
- wherein,
- Seqforward is GPR160 gene or a GPR160 gene fragment;
- Seqreverse is a nucleotide sequence that is substantially complementary to Seqforward;
- X is a spacer sequence located between Seqforward and Seqreverse, and the spacer sequence is not complementary to Seqforward or Seqreverse.
A dsRNA, wherein the dsRNA has the structure as shown in Formula II:
- wherein,
- Seq′forward is a RNA sequence or a sequence fragment corresponding to the Seqforward sequence;
- Seq′reverse is a sequence that is substantially complementary to Seq′forward;
- X′ is none, or a spacer sequence located between Seq′forward and Seq′reverse, and the spacer sequence is not complementary to Seq′forward and Seq′reverse,
- ll represents a hydrogen bond formed between Seq′forward and Seq′reverse;
- preferably, the sequence of GPR160 gene is shown in SEQ ID NO.:
- 1;
- more preferably, the sequence of GPR160 gene fragment is shown in SEQ ID NO.:
- 6 or SEQ ID NO.:
- 7.
A composition, wherein the composition contains an inhibitor or antagonist inhibiting GPR160 gene expression or antagonizing GPR160 receptor activity;
- preferably, the inhibitor includes:
- Small interfering molecules or antisense nucleotides that specifically interfere with GPR160 gene expression;
- or
- a biological or chemical ligand that specifically binds to a GPR160 gene-encoded protein.
A method for preparing dsRNA, including steps of:
- (i) preparing a construct expressing dsRNA, wherein the construct is double-stranded, and its sense or antisense strand contains the structure shown in Formula 1:
- SeqForward-X-SeqReverse Formula I
- wherein,
- Seqforward is a GPR160 gene or GPR160 gene fragment;
- Seqreverse is a nucleotide sequence that is substantially complementary to Seqforward;
- X is a spacer sequence located between Seqforward and Seqreverse, and the spacer sequence is not complementary to Seqforward or Seqreverse;
- (ii) transferring the construct in step (i) into a host cell, thereby forming dsDNA as shown in Formula II in the host cell through expression,
- wherein,
- Seq′forward is a RNA sequence or sequence fragment corresponding to Seqforward;
- Seq′reverse is a sequence that is substantially complementary to Seq′forward;
- X′ is none, or a spacer sequence located between Seq′forward and Seq′reverse, and the spacer sequence is not complementary to Seq′forward or Seq′reverse,
- ll represents a hydrogen bond formed between Seq′forward and Seq′reverse;
- preferably, the sequence of GPR160 gene is shown in SEQ ID NO.:
- 1;
- more preferably, the sequence of GPR160 gene fragment is shown in SEQ ID NO.:
- 6 or SEQ ID NO.:
- 7.
A method for inhibiting GPR160 gene expression, comprising contacting cells expressing GPR160 with an effective amount of a nucleic acid comprising the nucleotide sequence of SEQ ID NO.:
- 6 or SEQ ID NO.
Dependencies are inferred from the claim text.