Preparation of multi-hapten mutant g6pdh conjugates and their use for detection of multiple analytes
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsYi Feng Zheng, Yali Yang, Linda Hudson, Nihmat Morjana
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Abstract
The present invention relates to multi-hapten mutant G6PDH conjugates, methods of their preparation and their use for detection of multiple analytes. The compositions of the invention comprise different types of haptens (molecules) require to be immobilized into the G6PDH to make one multi-hapten-G6PDH conjugate. Both thiol and amine functional groups on G6PDH are utilized for immobilization of different haptens.
Claims11
1-11. (canceled)
A method for producing a composition, the composition comprising at least two distinct specific binding pair (sbp) members conjugated to a mutated glucose-6-phosphate dehydrogenase (G6PDH), wherein the mutation comprises the insertion or substitution of at least one cysteine amino acid residue and wherein at least a first specific binding pair member is conjugated to said mutated G6PDH via a thiol group on the cysteine and wherein at least a further specific binding pair member is conjugated to G6PDH via an amino group, the method comprising the steps of:
- conjugating a first specific binding pair member to mutant G6PDH via a thiol group;
- and then
- conjugating at least a further specific binding pair member to mutant G6PDH via an amino group.
(canceled)
Dependencies are inferred from the claim text.